laura chimenti origini calabresi

Previous studies showed that tyrosine hydroxylase or neurturin gene-modified cells transplanted into rats with Parkinson's disease significantly improved behavior and increased striatal dopamine content. View the profiles of people named Laura Chimenti. Intravenous allogeneic MSC infusion has proven to be safe in a phase-I trial in patients with acute MI. SMCs were isolated from HFCs using tissue-specific promoters and flow cytometry sorting. FROUNT was required for polarization of MASCs, resulting in clustering of CCR2 and reorganization of the cytoskeleton. Our results demonstrate that Dkk-1 is an LRP6 ligand and inhibits Wnt signaling by blocking Wnt-induced Fz-LRP6 complex formation. Labeled stem cells can be used to track migration and distribution in vivo by MRI. in osteogenic differentiation of adult stem cells and suggest that commitment of hMSCs into osteogenic or adipogenic lineages Allogeneic MSC injected prior to transplantation are immunogenic in this model because activated CD4+ and CD8+ cells emerged earlier in secondary lymphatic organs of MSC- and MSC/MMF-treated animals, compared to animals not treated with MSC. It was felt that international coordination between North America and Europe in clinical studies of ARDS was becoming increasingly important in order to address the recent plethora of potential therapeutic agents for the prevention and treatment of ARDS. Muscle regeneration and stem-cell fate were characterized. The use of nonautologous stem cells isolated from healthy donors for stem-cell therapy is an attractive approach, because the stem cells can be culture expanded in advance, thoroughly tested, and formulated into off-the-shelf medicine. Our findings indicate that Flk1+ cells can act as 'vascular progenitor cells' to form mature vessels and thus offer potential for tissue engineering of the vascular system. Results: To determine the usefulness of samples obtained by bronchoalveolar lavage (BAL) in establishing the diagnosis of ventilator-associated pneumonia, quantitative cultures of BAL and protected specimen brush (PSB) samples obtained via fiberoptic bronchoscope were compared in 42 patients with suspected ventilator-associated pneumonia. Incidence increased with age and was higher in males (2.1 vs 1.6 per 1,000 population). The LOD System identified from 1 to 3 levels of organ dysfunction for 6 organ systems: neurologic, cardiovascular, renal, pulmonary, hematologic, and hepatic. We have shown that basic fibroblast growth factor (bFGF) supplementation induces rapid proliferation of human umbilical vein endothelial cells (HUVEC), which is reduced upon removal of bFGF or by bFGF immunoneutralization. In the clinical study, serum levels of MCP-1 in 24 patients with acute myocarditis at the time of admission (165.2 +/- 55.8 pg/ml) were significantly (P = 0.0301) elevated compared with those of 20 healthy volunteers (61.8 +/- 10.7 pg/ml). 014011. The observation of chemokine dysregulation in human end-stage heart failure may represent a previously unknown mechanism involved in progression of chronic heart failure. In this review we are going to describe briefly the different cell types, their potential sources and characteristics and mechanism of action. Together, our data indicate that MSC pre-activate T cells, but co-treatment with MMF eliminates these T cells, decreases intragraft APC and T cell trafficking by inhibiting endothelial activation, and allows IFN-γ stimulation of suppressive MSC. The differentiation and paracrine action of MSC were observed after 2 weeks of MI. Emerging evidence suggests that the balance between IL-1 agonists and antagonists plays an essential role in a variety of cardiovascular conditions. Ejection fraction and left ventricular volumes determined by echocardiography and magnetic resonance imaging were exploratory efficacy end points. A cationic liposome was prepared as an efficient carrier for miRNA delivery. Also, the heterogeneity of patients with sepsis and ARDS is massive, and establish a target population or the stratification of the patients will help us to determine better the therapeutic effect of these cell therapies. Cytochalasin D had identical effects, whereas three other tyrosine kinase inhibitors did not. The cells were shown to have evidence of cardiac potential through the expression of green fluorescent protein under control of the Nkx2.5 promoter. Conclusion Nebulized heparin reduced pulmonary coagulopathy and inflammation without producing systemic bleeding, partly by modulating alveolar macrophages. Entrambi tipicamente calabresi, del catanzarese, del reggino e del vibonese, La Scala è di Lamezia Terme nel catanzarese e di Mileto nel vibonese con presenze nel reggino, Lascala è di Mileto nel vibonese e di Bianco nel reggino, potrebbero derivare dal nome del paese di Scala Coeli nel cosentino, forse il luogo d'origine dei capostipiti. After decades of research, and numerous pre-clinical and clinical trials, sepsis and ARDS remain without a specific and effective pharmacotherapy and essentially the management remains supportive. Here, also we elucidate the results of several pre-clicinical and clinical studies in ARDS and in sepsis and the future directions of these studies. The latter may have beneficial effects in patients with acute lung injury, affording reductions in pulmonary inflammation, lessened oxidative alveolar damage, and the regulation of innate immunity and host defenses by inhibiting the expression of inflammatory cytokines. We report that Dkk-1 is a high-affinity ligand for LRP6 and inhibits Wnt signaling by preventing Fz-LRP6 complex formation induced by Wnt. In the present study, we transplanted tyrosine hydroxylase and neurturin gene-modified bone marrow-derived mesenchymal stem cells into the damaged striatum of Parkinson's disease model rats. We conclude that precise intracuff pressure measurement is mandatory to prevent complications of over- or underinflation. For example, contributing factors in the progression of heart failure are both the loss of cardiomyocytes after myocardial infarction, and the absence of an adequate endogenous repair signaling. A new way to assess organ dysfunction in the intensive care unit. Patients under mechanical ventilation are at risk for several problems and complications, in particular ventilator-associated pneumonia (VAP). Furthermore, the hypoxia treatment led to significant migration and engraftment of CLK cells following intravenous infusion and significant functional effects including decreased infarct size, increased vascular growth, and improved cardiac function. Cytokines and chemokines (TNF-α, IFN-γ, IL-6, IL-12, PGE2, VEGF-α, Ang-1, KGF, IGF-1, PDGF-BB, and EPO) in culture medium of macrophages, MSCs and pMΦ were detected by ELISA and Real-Time-PCR. MSCs from rat femur, with or without HPC, were exposed to hypoxic conditions in cell culture (1% O(2) for 24h) and cell survival (by the LDH release assay and Annexin-V staining) was measured. Adult human mesenchymal stem cells are primary, multipotent cells capable of differentiating to osteocytic, chondrocytic, Interaction between endothelial cells and mural cells (pericytes and vascular smooth muscle) is essential for vascular development and maintenance. The positive outcomes confirm the efficacy of pb-/bm-fibrin clot, and bm-fibrin clot was proved to have much potential for tissue regeneration compared with pb-fibrin clot. The five lines of transgenic mice thus produced showed targeted expression of MCP-1 transcripts and protein in the adult heart muscle and pulmonary vein but not in skeletal muscle. Med. The results confirm the usefulness of the quantitative BAL culture (with a cut-off at 10(4) cfu/ml) for the diagnosis of ventilator-associated pneumonia. The present study critically tests hypothesis that hMSCs provide specific benefit directly to damaged ventricular myocytes. mRNA of MCP-1 increased in the hearts of EAM rats from days 15--27 as shown by quantitative reverse transcription-polymerase chain reaction. Interviews were done by a physician before and 12 weeks after the operation in regard to the impact of surgery on their sexual activities. Because vitamin C is safe and effective, it could be used to increase the yield and regenerative potential of ASCs. The biochemical and biological consequences of STAT serine phosphorylation are discussed. IL-1 signaling may also be an essential mediator in the pathogenesis of heart failure by suppressing cardiac contractility, promoting myocardial hypertrophy, and inducing cardiomyocyte apoptosis. The work cannot be changed in any way or used commercially. inserts, we found that stromal cells produce soluble mediators that increase TER and decrease TNFalpha secretion. ICU Scoring Group, Nebulized Heparin Attenuates Pulmonary Coagulopathy and Inflammation through Alveolar Macrophages in a Rat Model of Acute Lung Injury, The Optimal Intervention Time of Bone Marrow Mesenchymal Stem Cells in Ameliorating Cardiac Fibrosis Induced by Viral Myocarditis: A Randomized Controlled Trial in Mice, Cell therapy for the treatment of sepsis and acute respiratory distress syndrome, Intratracheal instillation of alveolar type II cells enhances recovery from acute lung injury in rats, Urinary Tissue Inhibitor of Metalloproteinase-2 and Insulin-Like Growth Factor-Binding Protein 7 for Risk Stratification of Acute Kidney Injury in Patients With Sepsis, Multiple mechanisms of SDF-1 promoting VEGF-induced endothelial differentiation of mesenchymal stem cells, Bone marrow-derived mesenchymal stem cells increase dopamine synthesis in the injured striatum, Transplantation of Bone Marrow Mesenchymal Stem Cells for the Treatment of Type 2 Diabetes in a Macaque Model, Effects of bone marrow mesenchymal stromal cells on gross motor function measure scores of children with cerebral palsy: A preliminary clinical study, Exposure to supernatants of macrophages that phagocytized dead mesenchymal stem cells improves hypoxic cardiomyocytes survival, Novel Role of NADPH Oxidase in Angiogenesis and Stem/Progenitor Cell Function, In Situ Articular Cartilage Regeneration through Endogenous Reparative Cell Homing Using a Functional Bone Marrow-Specific Scaffolding System, Current Status of Stem Cell Therapy for Sepsis and Acute Respiratory Distress Syndrome, Application of stem cell/growth factor system, as a multimodal therapy approach in regenerative medicine to improve cell therapy yields, Anti-inflammatory and immune modulatory properties of mesenchymal stromal cells, Concise Review: Mesenchymal Stromal Cells: Potential for Cardiovascular Repair, Hypoxia converts myogenic action of insulin-like growth factors into mitogenic action by differentially regulating multiple signaling pathways, Vascular endothelial growth factor (VEGF) as a key therapeutic trophic factor in bone marrow mesenchymal stem cell-mediated cardiac repair, Muscular Dystrophy Therapy by Nonautologous Mesenchymal Stem Cells: Muscle Regeneration Without Immunosuppression and Inflammation, Heart failure therapy mediated by the trophic activities of bone marrow mesenchymal stem cells: A noninvasive therapeutic regimen, Paracrine Mechanisms in Adult Stem Cell Signaling and Therapy, Pretransplant Infusion of Mesenchymal Stem Cells Prolongs the Survival of a Semiallogeneic Heart Transplant through the Generation of Regulatory T Cells, Integrin function: Molecular hierarchies of cytoskeletal and signaling molecules, Myocarditis induced by targeted expression of the MCP-1 gene in murine cardiac muscle, Myocardial expression of CC- and CXC-chemokines and their receptors in human end-stage heart failure, Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli, Vascular endothelial growth factor can signal through platelet-derived growth factor receptors, Adult Human Mesenchymal Stem Cell Differentiation to the Osteogenic or Adipogenic Lineage Is Regulated by Mitogen-activated Protein Kinase, Miyamoto S, Teramoto H, Coso OA, Gutkind JS, Burbelo PD, Akiyama SK, Yamada KMIntegrin function: molecular hierarchies of cytoskeletal and signaling molecules. MSC infusions, which makes the tolerogenic protocol applicable in a clinical setting. Large mononuclear cells in the myocardial interstitium were stained with an anti-MCP-1 antibody. Conclusion: Sexual dysfunction and reduction in serum level of sex hormones are common in patients before on-pump CABG and mostly get worse after surgery. ... Color images available online at in the final part of this review. We also discuss the coordination between different Wnt signaling cascades to precisely control MSC cell fate decisions, and we dissect the functional cross-talk of Wnt signaling that is known to occur with other growth factor signaling pathways. Since massive irreversible loss of cardiac myocytes occurs following myocardial injury, injection of human mesenchymal stem cells (hMSCs) has emerged as a promising therapeutic intervention. NADPH oxidase is one of the major sources of ROS in ECs and stem/progenitor cells, and is activated by various growth factors, cytokines, hypoxia, and ischemia. Vascular cells derived from Flk1+ cells can organize into vessel-like structures consisting of endothelial tubes supported by mural cells in three-dimensional culture. We use retrospective data from the hospital discharge database of all public and private hospitals in Catalonia (7,601,813 population), during the 5-year study period. In contrast, out of eight cytoskeletal molecules tested, only tensin coaccumulated. To examine the performance of the urinary biomarker panel tissue inhibitor of metalloproteinase-2 and insulin-like growth factor-binding protein 7 in patients with sepsis at ICU admission. Understanding these mechanisms may provide insight into NADPH oxidase and its mediators as potential therapeutic targets for ischemic heart and limb disease. ... MSC are multipotent cells that have been isolated from several tissues such as umbilical cord blood, placenta, adipose tissue, lung and bone marrow (25. Patients in 2011 were older (62.1 +/- 16.7 vs 64.9 +/- 14.9, p = 0.023) and more severe (APACHE II score 20.7 +/- 7.2 vs 22.4 +/- 7.9, p = 0.001). We also found that pretreatment of brown adipocytes with IL-1β or LPS led to a more pronounced (2–4-fold) induction of IL-6 transcripts upon whole 24 h time course of adrenergic stimulation. Mesenchymal stem cells (MSC) inhibit the immune response in vitro, and thus are promising candidate cells to promote acceptance of transplanted organs in vivo. Bone marrow mesenchymal stem cells (BMSCs) are self-renewing, multipotent cells that can migrate to pathological sites and thereby provide a new treatment in diabetic animals. The primary end point was incidence of treatment-emergent adverse events within 6 months. Here we show that Flk1+ cells derived from embryonic stem cells can differentiate into both endothelial and mural cells and can reproduce the vascular organization process. The transmigration of hematopoietic progenitor cells (HPCs) across vascular endothelium is a critical step in the homing of transplanted stem cells, but the molecular basis for this is unknown. Here we report that whereas IGF promotes myoblast differentiation under normoxia, it stimulates proliferation under hypoxia. The results obtained suggest that upon injury cells secrete a substance which interacts with collagen matrix to produce a homing agent. Although umbilical cord-MSCs are until now not particularly used as MSC source in clinical practice, accumulating evidences show that they may have a therapeutic advantage to treat several diseases especially autoimmune and neurodegenerative diseases. Consistent with cytokine release, flow cytometry analyses reveal that hMSCs also block the LPS- and IL-1β-dependent activation of cardiac transcription factor, NF-κB. Myocardial and vascular regeneration have been initially proposed as mechanisms of stem cell action. 935 Patients were included. The incidence of nosocomial pneumonia in mechanically ventilated patients can be significantly reduced by using a simple method that decreases the chronic microaspirations through the cuff of endotracheal tubes. We compared continuous data by ANOVA and categorical data by Chi-Square. R&D Scientist. Cell-growth assay (WST-8) and real-time PCR (COL1A1) were also performed for the detection of the potential of fibroblast proliferation. Statistical significance p≤0.05 is considered. Hypoxia has been considered to affect the properties of tissue stem cells including mesenchymal stem cells (MSCs). Free shipping for many products! Recent studies have shown that Wnts and their downstream signaling pathways play an important role in the self-renewal and differentiation of MSCs. We examined explanted hearts from ten patients with end-stage heart failure (all chambers) and in ten organ donors using an RNase protection assays and immunohistochemical techniques. Animal and preliminary human studies of adult cell therapy following acute myocardial infarction have shown an overall improvement of cardiac function. Background: Treatment with basic fibroblast growth factor increased proliferation and prevented myogenic differentiation, suggesting that basic fibroblast growth factor can be used to expand the population of undifferentiated HFCs to the large numbers needed for therapeutic applications. The objective of this study was to examine mRNA levels and cellular localisation of chemokines and chemokine receptors in human CHF. The area under the receiver operating characteristic curve (95% CI) of tissue inhibitor of metalloproteinase-2 and insulin-like growth factor-binding protein 7 was 0.84 (0.73-0.92) and 0.85 (0.76-0.94), in low and high nonrenal Sequential Organ Failure Assessment score subgroups. The long-term safety and efficacy of allogeneic MSCs for cardiac repair remain to be established. Based on the persistent immune activation with elevated circulating levels of chemokines in patients with congestive heart failure (CHF), we have hypothesised a pathogenic role for chemokines in the development of CHF. Although FGF receptor phosphorylation was not affected by LAU-8080, the bFGF-mediated prolonged phosphorylation, and activation of Erk-1 and -2 were attenuated. BMSC- induced T-cell suppression was still evident when BMSCs were added in culture as late as 5 days after starting of MLRs. Quantitative BAL and PSB cultures coincided in 76% of cases. (%) [95% CI] (268 (41.5%) [41.12-41.78] vs 537 (74.6%) [74.22-74.87] p <0.001), fluids and vasopressors (264 (43.7%) [43.25-43.05] vs 582 (80.9%) [80.45-81.25],p<0.001), central venous pressure >=8 mm Hg (136 (23.2%) [22.89-23.41] vs 336 (46.7%) [46.39-46.91],p<0.001), central venous oxygen saturation O2 >=70% (36 (6.2%) [5.94-6.39] vs 203 (28.2%) [27.94-28.39],p<0.001). U Misi e Natali nella tradizione popolare cosentina, Ad Amantea una delle scuole migliori della Calabria, Scuola: oltre 3.5 milioni di euro alla Calabria per la didattica digitale, Cittadella Regione Calabria intitolata a Jole Santelli, I presidi slow food festeggiano 20 anni, porte aperte nelle aziende calabresi, Eccellenze calabresi: il pecorino di Monte Poro, I paesaggi della Locride in una mostra fotografica al Parco archeologico. The level of sex hormones were generally decreased after operation but it was statistically significant only for estrogen (p-value=0.02). Infine, cerc a l’origine del pr ocesso de-generativo ne i costi di ada ttamento al contesto . We used mobilized peripheral blood CD34(+) selected cells and cultured bone marrow microvascular (BMECs) and human umbilical vein endothelial cells (HUVECs) to investigate the adhesion and transendothelial migration of HPCs. Treatment of these stem cells with osteogenic supplements resulted in a sustained phase of ERK activation from day 7 The present study indicates that collagen fragments can mediate the migration of bone marrow stromal cells. LPS provokes release of TNFα from nMCMs which is blocked by hMSCs grown in co- or transwell cultures. Dkk-1 binds neither Wnt nor Fz, nor does it affect Wnt-Fz interaction. Scribd is the world's largest social reading and publishing site. Apoptosis of implanted mesenchymal stem cells (MSCs) limits the efficiency of MSC therapy. Pb-/bm-fibrin clots were incubated in human osteoblast derived from mesenchymal stromal cells (MSCs) or human skin fibroblast. Alizarin red staining and real-time PCR (COL1A1, RUNX2) were performed for the detection of osteogenic potential. 190 patients who were admitted to the intensive care unit during a 33-month period and whose condition suggested the need for prolonged intubation (> 3 days). Serum MCP-1 levels of 8 fatal cases (371.8 +/- 145.2 pg/ml) were significantly (P = 0.0058) higher than those of 16 cases who survived (65.5 +/- 12.8 pg/ml). The work highlights a cardiac repair mechanism mediated by trophic cross-talks among the injected MSCs, bone marrow, and heart that can be explored for noninvasive stem cell therapy. About Blood cultures before antibiotics, broad-spectrum antibiotics and all resuscitation measures differences were not statistically significant. Estimation of endotracheal (ET) cuff pressure by finger palpation is one of the methods currently used in the clinical setting. Although traditional isolation of these cells by plastic adherence results in a heterogeneous composite of mature and immature cell types, MSCs do possess plasticity of differentiation and under appropriate in vitro culture conditions can be modified to adopt cardiomyocyte and vascular cell phenotypic characteristics. The hMSCs in lung upregulated expression of multiple genes, with a large increase in the anti-inflammatory protein TSG-6. Hypoxia suppresses basal and IGF-induced Akt-mTOR and p38 activity, whereas it enhances and prolongs IGF-induced Erk1/2 activation in a HIF-1-dependent fashion. Ball et al. The work highlights an important cardiac repair mechanism mediated by the myriad of trophic factors derived from the injected MSCs and local musculature that can be explored for non-invasive stem cell therapy. Several cell types are used in the last years for the treatment of both syndromes showing high efficiency. After 1 week of alginate culture, cell viability was >93%. MSCs cultured for 100days under hypoxic conditions were superior to those cultured under normoxic conditions in the ability to differentiate into the chondro- and adipogenic, but not osteogenic, lineage. To investigate the mechanisms underlying the beneficial effect of hypoxia preconditioning (HPC) on mesenchymal stromal cells (MSCs) and optimize novel non-invasive methods to assess the effect of biological interventions aimed to increased cell survival. | 74 collegamenti | Visualizza il profilo completo di Laura su LinkedIn e collegati The tyrosine kinase inhibitors herbimycin A or genistein blocked both the accumulation of 19 out of 20 signal transduction molecules and JNK- and ERK-mediated signaling. Mesenchymal stromal cells (MSCs) are present in adult tissues, including bone marrow and adipose, from which they can be easily isolated and cultured ex vivo. To read the full-text of this research, you can request a copy directly from the authors. Integrin receptors play important roles in organizing the actin-containing cytoskeleton and in signal transduction from the extracellular matrix. Using stromal cells in coculture with polarized ECs grown on, Clinical trials have documented beneficial effects of mesenchymal stromal cells from bone marrow and adipose tissue (ASCs) as treatment in patients with ischemic heart disease. The questions can be asked, what do MSCs do naturally and where is the MSC niche? Growing stem cells on Earth is very challenging and limited to a few population doublings. MSCs were isolated from bone marrow of mice and dead MSCs were harvested after 6h hypoxia. This finding indicates VEGF as a key therapeutic trophic factor in MSC-mediated myocardial regeneration, and demonstrates the feasibility of human MSC therapy using trophic factor-based cell-free strategies, which can eliminate the concern of potential stem cell transformation. Cultures of neonatal mouse ventricular cardiac myocytes (nMCM) were subjected to two distinct acute stress protocols; incubations with either endotoxin, lipopolysaccharide (LPS) or toxic cytokine, IL-1β. Quotidiano online di informazione e cultura sulla calabria, eventi, sport, politica, arte, tradizioni e spettacoli. Accurately assessing the probability of hospital mortality is a useful adjunct for clinical trials. Neurologic, cardiovascular, and renal dysfunction were the most severe organ dysfunctions, followed by pulmonary and hematologic dysfunction, with hepatic dysfunction the least severe. By using neutralizing monoclonal antibodies, transforming growth factor beta1 and hepatocyte growth factor were identified as the mediators of BMSC effects.

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